Chlorophyll Expression Varies with Development State in the Temperature-Sensitive ch4 Mutation of Melilotus alba.
نویسندگان
چکیده
The ch4 mutant of sweetclover (Melilotus alba) has previously been demonstrated to be temperature- and photoperiod-sensitive for the accumulation of chlorophyll. Pigment content of ch4 mutant leaves was examined as a function of trifoliolate mass, an index of leaf development. Inhibition of chlorophyll accumulation caused by increased growth temperature or decreased photoperiod can be attenuated during the earliest leaf development stages but is almost complete after the trifoliolates have reached 10 milligrams in size. Once this size is reached, the total amount of chlorophyll per trifoliolate remains constant, and the amount of chlorophyll per gram of leaf decreases as the leaf expands. We have evidence for several other sweetclover mutants with this general phenotype, all with alterations previously determined to be located in different genes as evidenced by complementation following genetic crosses. This mutant phenotype may represent lesions in the chlorophyll biosynthetic pathway or the assembly of the photosynthetic apparatus.
منابع مشابه
A Temperature-Sensitive Chlorophyll b-Deficient Mutant of Sweetclover (Melilotus alba).
The ch4 mutant of sweetclover (Melilotus alba) has previously been demonstrated to be partially deficient in chlorophyll and to have a higher ratio of chlorophyll a to b than normal plants. We were able to substantiate these findings when plants were grown at 23 degrees C and lower (permissive temperatures). However, when grown at 26 degrees C (nonpermissive temperature) the plants produced sma...
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Mutants of sweetclover (Melilotus alba) with defects in the nuclear ch5 locus were examined. Using thin-layer chromatography and absorption spectroscopy, three of these mutants were found to lack chlorophyll (Chl) b. One of these three mutants, U374, possessed thylakoid membranes lacking the three Chl b-containing pigment-protein complexes (AB-1, AB-2, and AB-3) while still containing A-1 and A...
متن کاملContents of Chlorophylls a and b in Chlorophyll-Deficient Mutants of Sweetclover
Leaf extracts from twelve chlorophyll-deficient mutants of sweetc10ver (Melilotus alba Desr_) were compared spectrophotometrically and chromatographically with extracts from normal plants to determine contents of chlorophylls a and b_ Total chlorophyll contents in cotyledons and leaves of all mutants were significantly lower than those in corresponding tissues of normal plants_ Chlorophyll cont...
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ورودعنوان ژورنال:
- Plant physiology
دوره 87 1 شماره
صفحات -
تاریخ انتشار 1988